Nuclear Binding of Steroid Receptors: Comparison in Intact Cells and Cell-Free Systems
نویسندگان
چکیده
منابع مشابه
Nuclear binding of steroid receptors: comparison in intact cells and cell-free systems.
In regulating gene expression in mammalian tissues, steroid hormones bind to cytoplasmic receptors and the resulting complex associates with the nucleus. In an attempt to study this nuclear association under more precisely controlled conditions, we examined the binding of receptor-[(3)H]steroid complexes to isolated nuclei. Two systems were used: the glucocorticoid-responsive hepatoma cell and ...
متن کاملinvestigation of single-user and multi-user detection methods in mc-cdma systems and comparison of their performances
در این پایان نامه به بررسی روش های آشکارسازی در سیستم های mc-cdma می پردازیم. با توجه به ماهیت آشکارسازی در این سیستم ها، تکنیک های آشکارسازی را می توان به دو دسته ی اصلی تقسیم نمود: آشکارسازی سیگنال ارسالی یک کاربر مطلوب بدون در نظر گرفتن اطلاعاتی در مورد سایر کاربران تداخل کننده که از آن ها به عنوان آشکارساز های تک کاربره یاد می شود و همچنین آشکارسازی سیگنال ارسالی همه ی کاربران فعال موجود در...
Steroid-binding receptors in fungi: implication for systemic mycoses
It has been shown that some of the mycotic infections especially systemic mycoses show increased male susceptibility and some steroids have been known to influence the immune response. Researchers found that some fungi including yeasts use "message molecules" including hormones to elicit certain responses, especially in the sexual cycle, but until recently no evidence was available to...
متن کاملMicroassay for nuclear binding of steroid receptors with use of intact cells from small samples of avian and human tissue.
A micro version of a nuclear binding assay to assess the biological activity of receptors for steroid hormones was developed for application to small (needle) biopsies of human tumors for the purpose of predicting responses to steroid therapy. This easier assay requires 10-fold less tissue than the original nuclear binding assay described for progesterone receptors in the avian oviduct, endomet...
متن کاملThyroid hormone action in cell culture: domonstration of nuclear receptors in intact cells and isolated nuclei.
Triiodothyronine and thyroxine induce a 3-fold increase in the rate of growth of GH(1) cells in culture. To study further the action of these hormones, we examined the binding of [(125)I]triiodothyronine and purified [(125)I]thyroxine to cellular fractions after incubation with intact cells in serum-free medium. High-affinity, low-capacity binding sites for the hormones were demonstrated in nuc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1973
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.70.12.3415